مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Antimicrobial Effects of Steroidal and Non-Steroidal Drugs Used in Asthma on Clinical Bacterial Isolates
Antimicrobial Effects of Steroidal and Non-Steroidal Drugs Used in Asthma on Clinical Bacterial Isolates
Elham MirarabRazi,1Hadi Bazzazi,2,*Hamid Reza Pordeli,3
1. Department of Medical Laboratory Sciences, Go.C., Islamic Azad University, Gorgan, Iran 2. Department of Medical Laboratory Sciences, Go.C., Islamic Azad University, Gorgan, Iran 3. Department of Biology, Go.C., Islamic Azad University, Gorgan, Iran
Introduction: Introduction: Asthma is a multifactorial inflammatory disease of the airways, often associated with altered microbial communities in the lower respiratory tract. While inhaled corticosteroids and bronchodilators are standard treatments for asthma, their potential direct antimicrobial activity has received limited attention. This study investigates the antibacterial effects of selected asthma medications against clinically relevant respiratory pathogens.
Methods: Methods: The antimicrobial activity of two corticosteroids (Beclomethasone and Fluticasone) and two bronchodilators (Salbutamol and Salmeterol) was assessed against Staphylococcus aureus, Pseudomonas aeruginosa, and Streptococcus viridans, using the agar diffusion method and broth microdilution to determine minimum inhibitory concentrations (MICs). Drug combinations were also evaluated for potential synergistic effects. A total of 39 clinical bacterial isolates were tested. Statistical analysis was performed using GraphPad Prism with t-tests.
Results: Results: Fluticasone and Salmeterol showed significant antimicrobial effects. Fluticasone inhibited the growth of S. aureus and S. viridans with an MIC of 31.25 µg/mL, while Salmeterol demonstrated inhibitory activity against P. aeruginosa with an MIC of 125 µg/mL. Notably, the Fluticasone–Salmeterol combination resulted in a statistically significant synergistic antimicrobial effect (p < 0.01) compared to either agent alone.
Conclusion: Conclusion: This study provides novel evidence that certain asthma medications, particularly Fluticasone and Salmeterol, possess direct antibacterial activity against key respiratory pathogens. These findings suggest a potential dual role for these drugs—not only in inflammation control but also in modulating the lung microbiota—highlighting a previously underrecognized aspect of asthma pharmacotherapy.