مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Effects of zinc oxide nanoparticles on pancreas oxidative stress pathway in diabetic rats
Effects of zinc oxide nanoparticles on pancreas oxidative stress pathway in diabetic rats
Mohamad Parsa Golbaghi,1,*Ebrahim Abbasi,2
1. Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran 2. Nutrition Health Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Introduction: Zinc oxide nanoparticles show potential antioxidant and glucose lowering effects. Hence, the aim of this experiment was evaluation the effects of zinc oxide nanoparticles (ZnO NPs) on pancreas oxidative stress pathways in diabetic rats.
Methods: In this experiment the male rats were purchased from Hamadan University of Medical Sciences and, divided in in 4 groups: (1) healthy control rats (C); (2) diabetic animals (D), (3)(4)(5) diabetic rats received 5, 7.5, and 10 mg/kg nanoparticles. For induction of diabetes, a single dose of Streptozotocin was injected into rats. Then the ZnO nanoparticles were administrated. In the end of experiment, the pancreas was removed and used for evaluation of oxidative stress markers. The oxidative stress pathway gene expression was determined by Real time PCR and the activity of these enzymes evaluated by ELISA.
Results: The levels of lipid peroxidation were reduced, and glutathione and total antioxidant capacity (TAC) were increased by zinc oxide nanoparticles in diabetic rats. Zinc oxide nanoparticles also increased the gene expression and activity of glutathione peroxidase, super oxide dismutase, and catalase in Wistar diabetic rats.
Conclusion: This experiment revealed that zinc oxide nanoparticles reduce oxidative stress in the pancreas by affecting the glutathione peroxidase, super oxide dismutase, and catalase.