Introduction: Breast cancer is one of the most common causes of cancer-related deaths and often develops resistance to conventional treatments. Therefore, innovative treatment approaches are essential. This study assessed the cytotoxic effects and the ability to induce apoptosis of 2-amino-7,7-dimethyl-5-oxo-1'-phenylmethyl-5,6,7,8-tetrahydrospiro [Chromene-4,4'-piperidine]-3-carbonitrile (PMPC), a novel pyrano-chromene derivative, on the MCF-7 breast cancer cell line.
Methods: MCF-7 cells were exposed to different concentrations of PMPC. An MTT assay was used to determine cell viability, while apoptosis was assessed through fluorescent microscopy and DNA fragmentation analysis.
Results: PMPC decreased MCF-7 cell viability in a dose- and time-dependent manner, achieving an IC50 of 60 μM after 72 hours. Observed morphological changes and DNA fragmentation provided evidence for apoptosis induction.
Conclusion: PMPC exhibits considerable cytotoxicity and promotes apoptosis, underscoring its potential as a promising candidate for further anticancer research.
Keywords: Apoptosis, MCF-7 cell, Pyranochromene, Breast cancer