مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Evaluation of the anti apoptotic effect of carotenoids isolated from the BM1 bacterial strain from mangrove forests in Iran on prostate cancer cell line (DU145)
Evaluation of the anti apoptotic effect of carotenoids isolated from the BM1 bacterial strain from mangrove forests in Iran on prostate cancer cell line (DU145)
Introduction: Mangrove ecosystems, characterized by extreme conditions such as high salinity, temperature fluctuations, tidal changes, and intense light, host diverse microbial communities that are promising sources of bioactive natural products. Among these, halophilic microorganisms, particularly bacteria, are known to produce valuable metabolites like carotenoids. These carotenoids possess antioxidant and anticancer properties, making halophiles an attractive target for pharmaceutical research. Prostate cancer, the second most common cancer in men after lung cancer, remains a leading cause of cancer-related mortality worldwide.This study aims to investigate the properties of a carotenoid extract derived from the bacterial strain Planococcus sp. BM1, isolated from the Haradher mangrove ecosystem in Iran. We focus on its anti-apoptotic effects and the modulation of BAX/BCL2 protein expression in DU145 prostate cancer cell lines.
Methods: The bacterial strain was cultivated for carotenoid production, followed by extraction using organic solvents. Phylogenetic identification was performed through DNA extraction, 16S rDNA PCR amplification, and electrophoresis. Subsequently, fibroblast and DU145 cancer cells were cultured to assess cell survival, apoptosis rates, and expression levels of apoptosis-related proteins using MTT assays, cell cycle analysis, and Western blotting techniques.
Results: Morphological and biochemical analyses classified the strain as Gram-positive cocci with creamy-orange pigmentation on nutrient agar. Phylogenetic analysis via 16S rDNA gene sequencing confirmed its classification within the Planococcus genus. The carotenoid extract demonstrated significant antiproliferative effects, reducing cell viability to approximately 50% at a concentration of 250 mg/mL after 48 hours. Apoptosis assays indicated a significant increase in both early (7.0 1.5 to 15.5 ± 2.6%, p < 0.05) and late apoptotic cells (4.3 0.7 to 28.2 ± 3.5%, p < 0.001). Cell cycle analysis revealed a significant accumulation in the G2 phase (p < 0.01), indicative of DNA fragmentation. Western blot analysis confirmed elevated levels of BAX expression in treated cells (p < 0.001). These findings suggest that the carotenoid extract from Planococcus sp. BM1 exhibits potent antiproliferative and pro-apoptotic effects on prostate cancer cells, underscoring its potential as a therapeutic agent.
Conclusion: The carotenoid extract produced by Planococcus sp. BM1 effectively induces apoptosis in DU145 prostate cancer cells while modulating key apoptotic proteins, highlighting its promise for future therapeutic applications.