مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Natural Coagulant Hydrogels: Optimizing Herbal Extract-Blended Polymers for Rapid Hemostasis and Wound Healing
Natural Coagulant Hydrogels: Optimizing Herbal Extract-Blended Polymers for Rapid Hemostasis and Wound Healing
sara farahbakhsh,1,*sadaf faez,2Dornika Goodarzi,3Mahsa Davoodi,4Saba Abidi,5Mobina Haji Hosseini,6
1. PhD Graduate in Biotechnology, Bu-Ali Sina University, Hamadan, Iran 2. 9th Grade Student, Salam Islam Private Middle School (First Cycle), Tehran, Iran 3. 9th Grade Student, Salam Islam Private Middle School (First Cycle), Tehran, Iran 4. 9th Grade Student, Salam Islam Private Middle School (First Cycle), Tehran, Iran 5. 9th Grade Student, Salam Islam Private Middle School (First Cycle), Tehran, Iran 6. 9th Grade Student, Salam Islam Private Middle School (First Cycle), Tehran, Iran
Introduction: This study explored hydrogels made from cinnamon, yarrow, green tea, and red pepper extracts for wound healing. Cinnamon (1%, 2%) reduced clotting time to 30s, while yarrow (0.5%) increased it to 60s (1% returned to 30s). Green tea and red pepper extended clotting to 90s and 150s, respectively. The hydrogels showed excellent coagulation, moisturizing, and antioxidant properties, demonstrating potential for natural wound treatment and drug delivery.
Methods: Uncontrollable bleeding due to trauma, surgery, or clotting disorders remains a critical medical challenge. Conventional methods often fail in severe cases, necessitating advanced hemostatic solutions. Biocompatible hydrogels, incorporating natural polymers like chitosan and alginate, offer promising alternatives due to their water absorption, drug delivery, and wound-healing properties. Plant extracts (yarrow, cinnamon, aloe vera) enhance these hydrogels with anti-inflammatory, antimicrobial, and antioxidant effects, promoting synergistic benefits. This study aims to develop an optimized hemostatic hydrogel using these natural compounds, evaluating its physicochemical properties and efficacy in lab and computational models. The results could lead to safer, more effective treatments for acute and chronic bleeding.
Results: Plant extracts (2 g each) were prepared in 100 mL distilled water at 60–70°C for 30 min, then filtered. Coagulation time (CT) was assessed via two methods: (1) in vitro assay (100 µL extract + 500 µL blood at 37°C, monitoring clot formation every 30 s) and (2) slide test (observing fibrin formation via needle probing). For hydrogel synthesis, 1% sodium alginate (with 1% glycerol) was ionically crosslinked with 3% CaCl₂, while 3% chitosan (dissolved in dilute acetic acid + 1% glycerol) was modified with 0.5% vanillin as a crosslinker. The hydrogels were formed by blending alginate and chitosan (70:30 ratio). Hemostatic efficacy was evaluated by comparing blood clotting times on hydrogel vs. control surfaces. Samples were stored at 4°C.
Conclusion: This study demonstrates that cinnamon, aloe vera, and borage extracts at various concentrations significantly reduce blood clotting time. Cinnamon and aloe vera at 1% and 2% concentrations shorten clotting time to approximately 30 seconds, while borage at 0.5% extends it to 60 seconds. Increasing borage to 1% decreases clotting time back to 30 seconds. Additionally, incorporating these extracts into hydrogels accelerates coagulation and exhibits desirable clotting properties. Overall, these herbal extracts and hydrogels show promise for developing plant-based hemostatic agents and advancing therapeutic applications for blood control.