مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Anti-migration and Apoptotic Induction Effects of Froriepia subpinnata Extract -Functionalized Silver Oxide (Ag2O) Nanoparticles on AGS gasteric cancer cells via Modulation of miR-34a and EGFR gene
Anti-migration and Apoptotic Induction Effects of Froriepia subpinnata Extract -Functionalized Silver Oxide (Ag2O) Nanoparticles on AGS gasteric cancer cells via Modulation of miR-34a and EGFR gene
Introduction: Froriepia subpinnata (Ledeb.) Baill. is a traditional Iranian medicinal herb with limited studies on its antioxidant and therapeutic effects. The poor bioavailability of its hydrophobic bioactive compounds restricts its clinical potential. To overcome this, silver oxide nanocomposites (Ag₂O@Fs NCs) were synthesized as carriers to enhance solubility and provide targeted delivery to gastric cancer cells.
Methods: Ag₂O@Fs nanocomposites were synthesized by integrating phytochemicals extracted from Froriepia subpinnata with silver oxide nanoparticles. The obtained nanocomposites were characterized using FE-SEM for morphology and particle size distribution, TGA-DTG for thermal stability, and FT-IR for functional group analysis.
For biological evaluation, AGS gastric adenocarcinoma cells were cultured and treated with increasing concentrations of Ag₂O@Fs NCs. Cell viability was assessed using the MTT assay, and the IC₅₀ value was calculated. To investigate molecular mechanisms, qRT-PCR was performed to quantify expression levels of miR-34a (apoptosis-related) and EGFR (migration-associated).
Results: Nanocomposites displayed spherical morphology (48.7 ± 12.3 nm) and stability up to 310°C.
MTT assay revealed dose-dependent cytotoxicity with an IC₅₀ of 41.45 µg/mL.
qRT-PCR showed significant upregulation of miR-34a (3.8-fold, P < 0.01) and downregulation of EGFR (0.4-fold, P < 0.05), indicating induction of apoptosis and inhibition of cell migration.
Conclusion: Ag₂O@Fs NCs act both as effective nanocarriers enhancing phytochemical solubility and as bioactive agents that modulate oncogenic pathways. Their combined effect suggests strong potential for inhibiting gastric cancer growth and metastasis, supporting further preclinical investigations.