مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
NF-kB regulates the migration of vascular smooth muscle cells after heparin and ibrutinib treatments.
NF-kB regulates the migration of vascular smooth muscle cells after heparin and ibrutinib treatments.
Nafiseh Shokri,1,*
1. Shahid Beheshti University of Medical Sciences
Introduction: The aim of the study was to examine the effect of heparin on the migration and proliferation of vascular smooth muscle cells (VSMCs) and its association with the expression levels of NF-kB and collagen 3. Additionally, the incorporation of heparin in VSMC cultures containing betulinic acid was studied.
Methods: 8 cell groups were cultivated and administered with the Heparin, Betulinic acid based on the viability and toxicity in 24-h and 48-h periods. The gene and protein expression levels were assessed by RT-qPCR and western blotting techniques. The VSMC migration was assessed by scratch test.
Results: Heparin (30 IU) significantly boosted (P < 0.05) NF-kB gene and protein expression levels, as well as VSMC migration over the exposure period.Heparin (5 IU and 15 IU) raised collagen 3 gene expression levels during both times. Incorporating heparin into cells with betulinic acid impacted collagen 3 expression levels.
Conclusion: The data showed that Heparin doses (5 IU and 15 IU) were proper for vascular smooth muscle cells based on NF-kB and collagen 3 expression levels.