مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Antibacterial Effects of Nanoemulsions Containing Extract and Essential Oil of Juniperus polycarpos
Antibacterial Effects of Nanoemulsions Containing Extract and Essential Oil of Juniperus polycarpos
Saba Hajipour,1Abolfazl Shakeri,2Rezvan Yazdian,3Maryam Hashemi,4Zahra Salmasi,5,*
1. School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 2. Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 3. Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran 4. Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 5. Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Introduction: Juniperus polycarpos is a native species of the Iranian plateau, known for the wide range of medicinal applications. The purpose of this study was the preparation and evaluation of antimicrobial activity of nanoemulsions containing the Juniperus polycarpos extract and essential oil against Gram-negative and Gram-positive bacteria.
Methods: The essential oil was extracted using steam distillation and analyzed by GC-MS. The extract was prepared through ethanol maceration. The nanoemulsions containing essential oil or extract were then prepared in various concentrations. variou properties of nanoemusions including the viscosity and thermal stability were assesses. The antimicrobial activity (MIC and MBC) was determined and time-kill assay was also directed to evaluate the antimicrobial effects of the formulations containing the extract or essential oil.
Results: DLS analysis showed that nanoemulsions containing 5% essential oil or extract had average particle sizes of 14 nm and 41 nm, respectively, with a negative surface charge. Electron microscopy images displayed that the nanoparticles were uniform and spherical. The MIC and MBC values of the essential oil against S. aureus were 31.25 and 250 μg/mL, respectively. The results also indicated that the nanoemulsion containing 5% essential oil significantly reduced microbial colonies at specified time points.
Conclusion: It was concluded that nanoemulsions of 5% Juniperus polycarpos essential oil had remarkable antimicrobial activity against S. aureus. Further studies can confirm the usage of this formulation in medical purposes, including topical application in skin disorders.