• Investigating the Effects of Synthetic Silver Nanoparticles with Echinophora platyloba on Breast Cancer Cells by Scratch Assay
  • Amirreza Hashemian,1,* Mohammad Foad Noorbakhsh,2 Amir Ali Heidari,3 Amirhosein Ekhlasi,4
    1. Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
    2. Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
    3. Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
    4. Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran


  • Introduction: Breast cancer is a significant disease with treatment challenges. Echinophora platyloba has been studied for its anticancer phytochemical compounds. Additionally, the anticancer effects of silver nanoparticles are well-documented. The green synthesis of silver nanoparticles with Echinophora platyloba may reduce adverse effects on normal cells and enhance the anticancer properties of silver nanoparticles
  • Methods: In order to evaluate the inhibitory effects of synthetic green nanoparticle with Echinophora platyloba on the migration of MCF-7 and NIH3 cells, the wound healing migration model was used. Scratches were made on a monolayer of NIH3T3 and MCF7 cell lines and treated with 100, 200, 400 μg/mL of nanoparticles containing and 5, 10 μg/mL of taxol for different time points. Migration was compared in both control and test by taking images at 2, 24, and 48 hour intervals.
  • Results: Green nanoparticles synthesized with Echinophora platyloba showed a significant reduction in cell migration at 200 and 400 μg/mL in MCF-7 cells after 24 hours of incubation compared to the control. After 48 hours, a significant reduction was observed in all 3 doses of 200, 100, and 400 μg/mL. Taxol, as a positive control, showed a concentration-dependent effect in different doses, so it can be concluded that the results of the nanoparticles were not random and, in line with Taxol, had an anti-metastatic effect on lung cancer cells.
  • Conclusion: It can be concluded that the nanoparticle coated with Echinophora platyloba extract has anti-metastatic effect and is a potential candidate to help in cancer treatment
  • Keywords: Breast Cancer, Silver Nanoparticle, Echinophora platyloba