مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Diagnostic Value of ELISA test in diagnosis of Brucellosis
Diagnostic Value of ELISA test in diagnosis of Brucellosis
Fatemeh Kourkinejad_Gharaei,1,*Reza Razzaghi,2Hadi Osuli,3Mansoureh Momen_Haravi,4Mohammad Javad Azadchehr,5
1. Department of Infectious diseases, Samen Hospital, Sirjan School of Medical Sciences, Sirjan, Iran 2. Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran 3. Student Research Committee, Kashan University of Medical Sciences, Kashan, Iran 4. Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran 5. Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran
Introduction: Background: Brucellosis is one of the most common zoonotic diseases in Iran, especially in western and central regions such as Kashan, which have one. Rapid and accurate diagnosis of this disease is vital due to the severe neurological and skeletal complications of the disease. It is generally recognized, 70% low and the need for a level 3 biosafety laboratory limits its use. In this regard, serological methods similar to ELISA have a special place in diagnostic projects due to their high specificity and usability.
Objective: The aim of this study is to determine the diagnostic value of ELISA test in brucellosis in patients referred to Akhavan Clinic in Kashan in 2023-2025.
Methods: Methodology: This case-control study was conducted on 47 patients with brucellosis (positive titer in all three agglutination tests, Wright, Combs Wright and 2-mercaptoethanol) and 57 healthy individuals. The samples were tested with ELISA kit (IBL Germany) and ELISA device to measure IgG and IgM. The data were analyzed with SPSS version 2 software and statistical tests including receiver operating characteristic (ROC).
Results: Results: ELISA IgM was obtained with a cut-off point of 3.0, a level of 71.9%, a specificity of 71.9% and a Rockwell coefficient of reactivity of 0.751. (95% confidence interval: 0.614-0.795) IgG ELISA had lower specificity than IgM. IgM ELISA also showed a better diagnostic performance than agglutination (p<0.0001).
Conclusion: Conclusion : IgM ELISA with acceptable specificity (70%) and diagnostic accuracy was studied as an efficient tool in the diagnosis of brucellosis in the studied population. This method can be suitable for traditional methods, especially in endemic areas such as Kashan where access to limited tests is limited, and it can be used alongside agglutination tests to reduce diagnostic errors and improve patient management.