مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Evaluation and Comparison of the Antioxidant Properties of Carvacrol and Satureja Khuzestanica Essential Oil in Free and Nanoliposome Forms
Evaluation and Comparison of the Antioxidant Properties of Carvacrol and Satureja Khuzestanica Essential Oil in Free and Nanoliposome Forms
hadis rahimi,1,*Golamreza Shahsavari,2Nahid Sarlak3,3Maryam Hormozi,4Masumeh Jalalvand,5
1. Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 2. Assistant Professor, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 3. Department of Chemistry, Faculty of Sciences, Lorestan University, Khorram Abad, Iran 4. Assistant Professor, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 5. Assistant Professor, Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Introduction: Today, diabetes is one of the leading causes of death worldwide, and it is predicted that by 2024, approximately 800 million people will be affected. Even with advances in treatment, 59% of patients do not receive the necessary care. In this disease, oxidative stress and increased inflammation lead to progression, and ultimately these factors lead to many problems, including vascular problems and beta-cell dysfunction associated with high levels of reactive oxygen species (ROS). Many pharmaceutical compounds, including natural antioxidants such as carvacrol and Satureja Khuzestanica essential oil, have been used in the past to reduce inflammation and oxidative stress, but the clinical use of these compounds is still limited due to their low stability and unfavorable bioavailability. To overcome the aforementioned problems, novel approaches such as the use of nanoparticles, such as nanoliposomes, as carriers that are manufactured at the nanoscale, are used. This technology increases the stability, cellular uptake, and controlled release of these compounds. In this study, the antioxidant potential of carvacrol and Satureja Khuzestanica essential oil in both conventional and nanoliposomal forms has been investigated and evaluated.
Methods: In this study, Satureja Khuzestanica essential oil was extracted by steam distillation, and carvacrol was purchased from Sigma. Then, nanoliposomes were prepared by the thin-layer hydration method, and carvacrol and savory essential oil were loaded into them; thus, carvacrol nanoliposomes and savory essential oil nanoliposomes were prepared. Then, using DPPH (2,2-diphenyl-1-picrylhydrazyl) solution, the antioxidant activity of savory and carvacrol nanoliposomes was calculated, and the IC50 value (concentration required to inhibit 50% of DPPH radicals) was calculated using the linear formula for each. Vitamin C was used as a positive control in this experiment.
Results: In this study, IC50 values were calculated for carvacrol 201.03 and free Satureja Khuzestanica essential oil 189.03 μg/mL. While IC50 for Satureja Khuzestanica nanoliposomes was 130.36, and IC50 for carvacrol nanoliposomes was 168.49 μg/mL. Vitamin C, which was present as a positive control in this test, showed IC50 value of 93.67 μg/mL, indicating its strong antioxidant capacity.
Conclusion: According to the results presented in this study, carvacrol nanoliposomes and Khuzestani savory essential oil were more efficient in terms of antioxidant capacity than the free forms due to their greater dispersion in solution and the use of phospholipid in the preparation stage of nanoliposomes, which reacts with free radicals and reduces them, increasing their antioxidant effect.