مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
A Review on the Application of Stem Cell-Seeded Silk Fibroin Scaffolds for Dermal Regeneration
A Review on the Application of Stem Cell-Seeded Silk Fibroin Scaffolds for Dermal Regeneration
Sana Rahimian,1,*Hossein Najafi,2
1. Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran 2. Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Introduction: The reconstruction of extensive skin defects remains a significant clinical challenge. This review aims to provide a comprehensive evaluation of recent studies in the field of tissue-engineered skin substitutes, with a particular focus on the synergistic combination of silk fibroin (SF) scaffolds and mesenchymal stem cells (MSCs) for achieving high-quality skin regeneration.
Methods: To this end, a systematic search was conducted in databases including PubMed, Scopus, and Web of Science for articles published from 2020 onward. The search keywords included "silk fibroin", "mesenchymal stem cells", "tissue engineering", "skin regeneration" and "regenerative medicine." Articles were selected based on their emphasis on preclinical and laboratory evaluations of SF-MSC composite structures.
Results: A recurring key finding is that MSCs cultured on silk scaffolds significantly accelerate wound closure in animal models by upregulating critical angiogenic factors such as VEGF and bFGF. Multiple studies demonstrate that the porous architecture of silk sponges produced by freeze-drying facilitates deep cell infiltration and angiogenesis, while electrospun nanofiber layers form a stable mechanical barrier on the outer surface.
Conclusion: Consequently, the combination of silk fibroin’s biocompatibility and tunable mechanical properties with the high regenerative secretory potential of mesenchymal stem cells represents one of the most advanced approaches in the field of skin substitutes. Future research should focus on the mass production of these constructs for the development of strategies for complete restoration of complex skin appendages.