• Bioremediation of VOCs and DOC: Using Algae-Based Systems
  • Shima Mokhtari Garakani,1,* Soha Mokhtari Garakani,2
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  • Introduction: The influence of Dissolved organic carbon (DOC) and Volatile Organic Compounds (VOCs) on both human health and the environment is significant and cannot be overlooked. VOCs not only play a role in photochemical processes but also directly influence physiological and disease- related mechanisms (1,2). Research indicates that the presence of VOCs correlates with respiratory conditions such as asthma and chronic obstructive pulmonary disease (COPD) (3). Continuous presence of VOCs has been linked to neurological impairments, heart-related illnesses, and premature deliveries. Additionally, about one-third of people may face an increased risk of cancer due to VOCs. Meanwhile, DOC have been implicated in raising the likelihood of cancers—especially those affecting the bladder, colon, and liver—and are associated with reproductive health issues, including infertility, a higher risk of miscarriage, developmental problems in fetuses, and complications involving the liver and digestive systems (4,5,6,7). Using algae-based systems is one of the reliable and green solutions for pollution reduction.
  • Methods: This approach simultaneously reduces pollution and generates valuable biomass. In algae-based methods—whether utilizing microalgae or macroalgae—several key mechanisms are involved: (1) adsorption and bioaccumulation of organic compounds on the cell membrane and within the biofilm matrix, (2) biodegradation or biotransformation carried out by the algae themselves or by symbiotic bacteria within their surrounding phycosphere, and (3) gas transfer or phase absorption in liquid-gas systems such as photobioreactors, which enable the metabolism of VOCs dissolved in the aqueous phase(8,9). • For water pollutants such as DOC, mixotrophic algae—particularly species like Galdieria sulphuraria—have demonstrated the ability to effectively reduce DOC and BOD levels. These algae have also shown remarkable efficiency at the pilot scale. This means that algae not only remove carbon but also convert it into usable biomass (10). • According to the research by Lebrero et al. the alga Chlorella vulgaris has been found effective in the removal of VOCs such as toluene and benzene (12). In another study, Hank et al. reported that Galdieria sulphuraria has a high capacity for DOC removal and can thrive under harsh environmental conditions (13). Olivia et al. also stated that a species of Scenedesmus sp. is effective in eliminating organic pollutants, including VOCs (14). Similarly, Satya et al. presented comparable findings by Chlamydomonas reinhardtii (15). In another report, Sia and collaborators introduced an algal–bacterial consortium aimed at enhancing the removal of organic compounds and improving treatment efficiency (16).
  • Results: In the treatment of DOC, Galdieria sulphuraria and Chlorella sorokiniana have demonstrated a direct and favorable role (17, 18). Also, In the case of Chlorella sp. D046 and Navicula sp., water quality improvement has been reported, although the main focus of the study was on the growth of the algae (19). • . Experiments conducted on photobioreactors and algae-bacteria aerated reactors have shown that the removal efficiency of compounds such as toluene can be very high, with some integrated systems achieving up to approximately 99% removal. Meanwhile, the CO₂ produced from the degradation process can be absorbed by algae and converted into biomass, thereby supporting the concept of a circular economy. These findings have been confirmed through both pilot-scale and field studies (8). • One significant advancement is the use of algae–bacteria consortia; in this system, bacteria are capable of breaking down hydrophobic or more complex compounds and producing intermediate products that algae can utilize. As a result, the efficiency of VOC removal is significantly enhanced. Recent studies have introduced "bottom-up" approaches for designing and screening such consortia (11). • Gas–liquid mass transfer for highly volatile VOCs, toxicity at high concentrations (which can inhibit both algae and bacteria), and industrial-scale issues such as lighting, biomass harvesting, and costs remain practical obstacles. Circular economy considerations—such as utilizing wastewater or flue gas CO₂ as resources—and integration with systems like MBBR, bio trickling filters, and photobioreactors have shown promising solutions (9, 10). • Integrated algal–bacterial systems in photobioreactors (or hybrid configurations such as MBBR–PBR) have proven to be effective for the simultaneous removal of VOCs and DOC. These systems not only reduce contaminants but also facilitate biomass production and CO₂ sequestration. Nevertheless, further advancements in mass transfer, strain optimization, and reactor engineering are essential for successful industrial-scale applications (11).
  • Conclusion: In conclusion, algae-based technologies for the removal of VOCs and DOC offer a sustainable and reliable solution, making them a promising component of future green remediation strategies.
  • Keywords: Dissolved organic carbon (DOC), VOCs