مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Fabrication of Bio -adhesive gel for Tissue Regeneration : In Vitro and In Vivo study
Fabrication of Bio -adhesive gel for Tissue Regeneration : In Vitro and In Vivo study
Farzaneh Chehelcheraghi,1,*
1. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran, fr.Chehelcheraghi@gmail.com, Chehelcheraghi.farzaneh@sbmu.ac.ir https://orcid.org/0000-0002-5796-9184
Introduction: Using natural compounds can overcome the disruption of traditional sutures and the problems of tissue adhesives in the wound healing process. The biomedical market has seen many effective skin products, but many of them still have limited use. This research was to use both herbal essential oil and gels to fabricate and study a new oleoresin essential oil of wild pistachio tree Tragacanth resin xerogel-like Bio-adhesive for Tissue Regeneration. Its low stiffness, scaffold-based matrix, due to the gel part, can facilitate wound healing.
Methods: Different concentrations of resin (6-9 w/v) and essential oils (0.2 -1 w/v) were blended according to the response surface methodology using the central composite design. The attachments were fabricated using the freeze-drying technique. These were assessed physically and biologically, including cell viability, cell attachment, SEM, FTIR techniques, and an in vivo mouse incision injection model.
Results: Tragacanth resin is an adhesive agent, a glue. The gel part could affect the physical properties of attachments, including FTIR, the strong peak at 1638.57 cm-1, and Particle size, including SEM Mean ±Std Dev; 47 ±0.89 µm. Also demonstrated no cytotoxicity towards H-Fib cells, with 80% cell viability and good attachment, and cell concentrations justified by the gel content. Also showed good in vivo biocompatibility.
Conclusion: Fabricated Bio-adhesive gel-like attached with essential oil of wild pistachio content can be a good candidate for regeneration-skin-based surgical purposes. However more in vitro and in vivo revisions are mandatory to adjust the herbal essential oil resin ratio.