• Alamandine Mitigates Inflammation and Oxidative Stress in OVA-Induced Asthma: A Novel Therapeutic Approach
  • Ali Abbasi,1 Ava Soltani Hekmat,2,* Kazem Javanmardi,3 Zahra gholami,4
    1. student research committee, Fasa university of medical sciences, Fasa, Iran
    2. Department of Physiology, Fasa university of medical sciences, Fasa, Iran
    3. Department of Physiology, Fasa university of medical sciences, Fasa, Iran
    4. student research committee, Fasa university of medical sciences, Fasa, Iran


  • Introduction: Asthma is a chronic disease with eosinophilic inflammation and oxidative damages leading to airway obstruction. Alamandine (Ala), a new member of the renin-angiotensin system (RAS), is an important peptide with antioxidant and anti-inflammatory capacities. The current study is devoted to evaluating Ala effects on the attenuation of inflammation and oxidative stress in lung tissue in a rat model of ovalbumin-induced asthma.
  • Methods: Bronchial asthma was induced by sensitization with Ovalbumin (OVA) intraperitoneally on day 0, 7 and 14. Following sensitization, rats were challenged with inhalation of a 1% ‎OVA solution using a nebulizer for 30 minutes daily from days 22 to 28. ‎Ala was administered via mini-osmotic pumps at a dosage of 50 μg ‎Ala/kg/day from day 15 to day 28. ‎. Following the final OVA challenge, mice were euthanized, and serum, bronchoalveolar lavage fluid (BALF), and lung tissue were collected for analysis. Various parameters, including eosinophil count, Th2-mediated inflammatory cytokines, proinflammatory cytokines, oxidative stress biomarkers, and NF-κB levels in BALF, along with IgE levels in serum, were evaluated.
  • Results: Administration of Ala resulted in reduced eosinophil count and decreased levels of IL-4, IL-5, IL-13, TNF-α, IL-6, IL-1β, and NF-κB in BALF. Additionally, Ala significantly lowered IgE levels in serum. Histopathological examination revealed suppressed lung inflammation, bronchial wall thickening, and alveolar edema in OVA-induced asthmatic mice treated with Ala. Moreover, Ala treatment led to a notable decline in malondialdehyde levels, accompanied by increased superoxide dismutase activity and glutathione peroxidase levels.
  • Conclusion: These findings underscore the therapeutic potential of Ala in asthma treatment by suppressing Th2-mediated cytokines and mitigating inflammation and oxidative stress associated with asthma. In summary, our findings suggest that Ala treatment holds promise as a novel and effective therapeutic approach for addressing OVA-induced asthma
  • Keywords: Asthma - Alamandine - ovalbumin