• Investigation and comparison of the effectiveness of using two drugs, an antibiotic and an anti-inflammatory, simultaneously in a nanocomposite formulation in improving skin wounds compared to using each drug alone in a laboratory mouse model.
  • Farya Mirzaei,1,* Negar Motakef Kazemi,2 Nasser Mohammadpour Dounighi,3 Mostafa Saffari,4 Sepideh Arbabi Bidgoli,5
    1. Department on Medical Nano Technology, TeMS.C., Islamic Azad University.
    2. Department on Medical Nano Technology, TeMS.C., Islamic Azad University.
    3. Department of venomous of animal, Razi Vaccines and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO) .
    4. Department of pharmaceutics, Islamic Azad University.
    5. Department of Toxicology and Pharmacology, Islamic Azad University, , .


  • Introduction: Chronic wound treatment, especially skin wounds, as an unpleasant complication and major challenge in the field of therapy and medicine, has always been a focus of attention. Drug delivery through the skin is one of the challenging issues in preparing and formulating drugs with the ability to overcome these problems due to various difficulties in local drug delivery, including multiple barriers, poor absorption, and the inability to use multiple drugs simultaneously in one site. The use of nanostructures and nanoparticles is recognized as an effective approach due to their particle size, the ability to apply various modifications in preparing nanostructures, and the capacity and capabilities of these particles in loading and targeted release. In this article, the results of using a nanocomposite containing two drugs—tetracycline (an antibiotic) and indomethacin (an anti-inflammatory)—in ointment formulation are discussed and compared with ointments containing each drug alone as well as the nanocomposite alone, in the healing and improvement of skin wounds in a Balb/c laboratory mouse animal model.
  • Methods: In this study, laboratory mice were divided into different groups and, after two days of being kept under identical environmental conditions and feeding, were assigned to various groups. For each group, after shaving the fur on the animals' lower backs, a wound of 1 square centimeter was created, and the intended drug was immediately applied as an ointment on the wound. The animals were monitored for one week, during which the wound area and the mice's weight were measured daily. Blood samples from the mice in all groups were collected on the last day, and tests measuring inflammatory and anti-inflammatory factors IL-10 and INF-γ were conducted. The results were analyzed based on the effectiveness levels.
  • Results: The results of this study were as follows: all mice in all groups showed signs of improvement after one week. Only one mouse in the control group died. No signs or symptoms of infection appeared in any group. The size of the wound scar was examined in all mice after one week. Based on the results and the healing speed, the group treated with the nanocomposite containing both drugs and indomethacin showed faster recovery than the other groups. The healing rate in the nanocomposite and tetracycline group was also higher than the control group but lower than the other two groups. The results of the IL-10 and INF-γ factor levels also supported these findings.
  • Conclusion: According to the obtained results, it is observed that the presence of anti-inflammatory agents plays a very beneficial and effective role in the wound healing process. The presence of the antibacterial agent also aids this process by preventing the occurrence and growth of microbial factors, thereby accelerating healing. Additionally, the results show that the presence of both agents together not only speeds up wound healing but also enhances tissue repair at the site and promotes hair regrowth in the examined area within a short period.
  • Keywords: Nano composite, simultaneously, antibiotic and anti-inflammatory, skin wounds, laboratory mouse.