مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Comparative study of the antimicrobial effects of ethanolic and acetonic extracts of Sinapis alba seed and Citrus sinensis peel against Staphylococcus aureus and Pseudomonas aeruginosa in a burn infection model
Comparative study of the antimicrobial effects of ethanolic and acetonic extracts of Sinapis alba seed and Citrus sinensis peel against Staphylococcus aureus and Pseudomonas aeruginosa in a burn infection model
Nader Kazemi,1Kimia Asi ebrahimi,2,*Mahya Mohseni,3
1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran 2. Department of Biology, Islamic Azad University., Zanjan Branch, Zanjan, Iran1 3. Department of Biology, Islamic Azad University., Zanjan Branch, Zanjan, Iran1
Introduction: The growing problem of antibiotic resistance and the limited success of common treatments in controlling some bacterial infections make it important to search for natural and effective alternatives or supportive options. This study aimed to compare the antimicrobial effects of ethanolic and acetonic extracts of Sinapis alba seeds and Citrus sinensis peel against two resistant bacteria in laboratory tests and in an animal burn infection model.
Methods: Extracts were prepared by maceration using ethanol and acetone. Antimicrobial activity was tested by measuring the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using serial dilution in 24-well plates. For the in vivo test, a mouse burn model infected with Pseudomonas aeruginosa (5×10^5 CFU/mL) was used. The effect on Staphylococcus aureus was evaluated in Mueller–Hinton medium.
Results: Both plants showed clear antimicrobial activity. For Sinapis alba seed, the MIC/MBC values were 3.9/7.8 mg/mL (ethanolic extract) and 15.6/31.2 mg/mL (acetonic extract). For Citrus sinensis peel, both extracts had an MIC of 3.2 mg/mL and an MBC of 7.8 mg/mL. In the animal model, the Citrus sinensis peel extract reduced inflammation and bleeding at the wound site. In general, ethanolic extracts were effective at lower concentrations.
Conclusion: The results suggest that plant extracts, especially ethanolic ones, have good potential in controlling resistant bacteria and improving burn wound infections. They may be useful in developing natural treatments against resistant pathogens.