مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Pan-cytokeratin Expression of Mesenchymal Stem Cells Cultured on Chitosan/Polyvinyl Alcohol Hydrogel Containing Caviar Extract
Pan-cytokeratin Expression of Mesenchymal Stem Cells Cultured on Chitosan/Polyvinyl Alcohol Hydrogel Containing Caviar Extract
Fatemeh Sadat Seyedi,1Fereshteh Sharifi,2Shiva Irani,3Seyed Mohammad Atyabi,4,*
1. Department of Biology, SR.C., Islamic Azad University, , 2. Department of Biology, CT.C., Islamic Azad University 3. Department of Biology, SR.C., Islamic Azad University 4. Department of Pilot Nano biotechnology, Pasteur Institute of Iran
Introduction: The development of wound dressings with optimal properties is crucial for accelerating the wound healing process. The objective of this study is to investigate the expression of the Pan-cytokeratin protein in stem cells cultured on a hydrogel-based wound dressing synthesized from chitosan (CS), polyvinyl alcohol (PVA), and caviar extract (Ce).
Methods: The CS/PVA/Ce hydrogel was synthesized using a freeze-drying method, and its structural properties were evaluated by scanning electron microscopy (SEM). The biocompatibility and metabolic activity of adipose tissue-derived stem cells (ADSCs) seeded on the optimized hydrogels were assessed after 24 hours using a MTT assay. The expression of Pan-cytokeratin protein was evaluated by immunohistochemistry (ICC) in the cells treated with the hydrogel.
Results: The CS/PVA/Ce hydrogel was synthesized using a freeze-drying method, and its structural properties were evaluated by scanning electron microscopy (SEM). The biocompatibility and metabolic activity of adipose tissue-derived stem cells (ADSCs) seeded on the optimized hydrogels were assessed after 24 hours using a MTT assay. The expression of Pan-cytokeratin protein was evaluated by immunohistochemistry (ICC) in the cells treated with the hydrogel.
Conclusion: According to the obtained results, the designed CS/PVA hydrogel containing caviar extract illustrated great potential for inducing Pan-cytokeratin protein expression and wound healing application.