• Evaluation of the Antimicrobial Effects of Ethanolic and Acetonic Extracts of Orange and Quince Peels on Burn Infections Caused by pseudomonas aeruginosa in Mice
  • Nader Kazemi,1,* Mahdieh Rezaie,2 Mahya Mohseni,3
    1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran
    2. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran
    3. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran


  • Introduction: Pseudomonas aeruginosa is a major cause of hospital-acquired and drug-resistant infections. With increasing bacterial resistance to chemical drugs, natural compounds with antimicrobial properties, such as plant extracts, have attracted attention. This study investigated the antimicrobial effects of ethanolic and acetonic extracts of orange and quince peels on burn infections caused by Pseudomonas aeruginosa in a mouse model.
  • Methods: After collecting and identifying the fruit peels, ethanolic and acetonic extracts were prepared at specific concentrations. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using serial dilution in nutrient broth. A bacterial suspension of P. aeruginosa (5×10^5 cfu/ml) was inoculated and treated with the extracts at MBC concentrations
  • Results: The results showed strong antimicrobial activity of both orange and quince peel extracts. Ethanolic extract of quince showed stronger effects than the acetonic one, with faster wound healing and reduced bleeding in treated mice. Orange peel extracts also demonstrated considerable efficacy in enhancing the healing rate.
  • Conclusion: Ethanolic and acetonic extracts of orange and quince peels possess effective antimicrobial properties in the treatment of burn infections caused by Pseudomonas aeruginosa. Notably, the ethanolic extract of quince had the highest effectiveness in wound healing and bleeding reduction, suggesting its potential as a natural and effective treatment for resistant infections.
  • Keywords: pseudomonas aeruginosa, Plant extract, Burn infection, Orange peel, Drug