• Examining the gene expression profile of colorectal cancer and functional prediction of some Long Non-Coding RNAs
  • Ahmad RezaeiNesab,1 Seyed Jalal Zargar,2,*
    1. Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
    2. Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran


  • Introduction: In 2020, with an estimated 1.93 million new cases (10%) and 0.94 million deaths (9.4%), colon cancer (CRC) is the third most diagnosed cancer and the second leading cause of cancer deaths among 36 types Cancer is investigated. Although it has been shown that the changes in the expression of some long chain non-coding RNAs play a significant role in the pathogenesis of various cancers such as CRC as key pathogenic factors, but the form of the expression changes as well as the pathogenic molecular mechanisms Many of these genetic factors in CRC still remain unknown.
  • Methods: Large-scale RNA sequencing data were obtained from the Cancer Genome Atlas database. Differentially expressed lncRNAs were identified by bioinformatics analysis. After identifying potential mRNA targets of 4 lncRNAs related to overall survival (OS), we performed a stepwise analysis of these targets through GO and KEGG. Using tissue samples from our own patients, we also confirmed certain analytical results using quantitative real-time PCR (qRT-PCR).
  • Results: Upregulation of TMEM105 expression was significantly correlated with TNM stage, poor survival and tumor recurrence in patients with CRC, and acted as an independent prognostic factor for tumor recurrence.
  • Conclusion: Our study elucidated that TMEM105 acted as an oncogene in CRC, which might serve as a novel target for CRC diagnosis and therapy.
  • Keywords: Colon cancer, LncRNAs, TMEM, Overall survival