مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Thymol as a Natural Adjuvant to Improve Cefotaxime Efficacy Against Acinetobacter baumannii
Thymol as a Natural Adjuvant to Improve Cefotaxime Efficacy Against Acinetobacter baumannii
Tarannom Mohammadi,1Mina Shirmohammadpour,2Sajjad jafari,3Bahman Mirzaei,4,*Mahdieh Hassani,5
1. Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran 2. Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran 3. Department of Microbiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, West Azerbaijan, Iran 4. Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran 5. Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Introduction: This study aimed at the antimicrobial effects of thymol/cefotaxime on Acinetobacter baumannii (A. baumannii) bacteria.
Methods: Antimicrobial effects of thymol/cefotaxime were performed first individually and then combined on A. baumannii ATCC19606 by the MIC-MBC method. Therefore, the antimicrobial effects of the compounds that had a synergistic impact were performed on eighteen clinical strains using the MIC-MBC method. The identification of chemical bonds, functional groups, and molecular interactions of the mentioned compounds was investigated using an FTIR device. Checkered method, time killing curve, and biofilm inhibition on A. baumannii ATCC19606, investigation of cytotoxicity on red blood cells (RBCs) by hemolysis method and human skin fibroblast cells (Ffk) by MTT method were performed. thymol/cefotaxime had synergistic effects.
Results: The study's findings demonstrated that when applied to A. baumannii ATCC19606, the antimicrobial activities of thymol, cefotaxime, and thymol/cefotaxime (A1 compound) were, respectively, 256, 128, and 512/128 (FICI: 1 µg/ml). The A1 compound exhibited antibacterial activity of 1024-512/256-128 µg/ml on clinical strains of A. baumannii, respectively. Compared to the individual modes, the combined mode had a longer time curve for eliminating A. baumannii. Examination with FTIR showed that these two compounds have C=C conjugated, C≡C compound. Thymol, cefotaxime, and other chemicals have biofilm inhibition rates of 29.69%, 16.28%, and 39.28%, respectively against A. baumannii bacteria. The toxicity of thymol, cefotaxime, and A1 compound against human RBCs were 36.12, 8.33, and 8.38, and against human Ffk cells were 19.66, 7.08, and 9.03 respectively.
Conclusion: This study proved that the thymol/cefotaxime combination could become one of the new drugs for treating A. baumannii infections due to its high antimicrobial effects and low toxicity.