مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Overcoming drug resistance: thymol and cefotaxime synergy against Acinetobacter baumannii and Klebsiella pneumoniae
Overcoming drug resistance: thymol and cefotaxime synergy against Acinetobacter baumannii and Klebsiella pneumoniae
Arman Taran,1,*Mina Shirmohammadpour,2Sajjad Jafari,3Bahman Mirzaei,4
1. Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran 2. Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran 3. Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran 4. Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Introduction: This research evaluates the combined antimicrobial effects of thymol and cefotaxime against Acinetobacter baumannii and Klebsiella pneumoniae.
Methods: The antimicrobial effects of thymol and cefotaxime were evaluated individually and in combination against K. pneumoniae ATCC 100031 and A. baumannii ATCC 19906 using the MIC-MBC method. Subsequently, the synergistic antimicrobial effects of these compounds were tested on ten clinical strains employing the MIC-MBC method. Chemical bonds, functional groups, and molecular interactions were analyzed using an FTIR device. Additional experiments included the checkered method, biofilm inhibition assays targeting K. pneumoniae and A. baumannii, as well as cytotoxicity tests on RBCs through hemolysis and Ffk using the MTT method. Synergistic effects of thymol and cefotaxime investigated, and the outcomes of the tests were compared between the two bacterial species.
Results: The antimicrobial effects of thymol/cefotaxime were more effective against K. pneumoniae compared to A. baumannii in both clinical and ATCC strains. FTIR analysis revealed the presence of bonds. A unique band, C=C conjugated and C≡C, highlighted the interaction between thymol and cefotaxime. The biofilm inhibition efficacy of thymol/cefotaxime was higher for K. pneumoniae than for A. baumannii. The cytotoxicity assessment revealed that the synergistic compounds demonstrated comparable effects on RBCs and human Ffk cells, with lower toxicity compared to Triton X-100.
Conclusion: This study found that thymol/cefotaxime demonstrated superior antimicrobial and anti-biofilm effects against K. pneumoniae compared to A. baumannii. With further research, this compound holds potential to be developed into a novel therapeutic option for patients.