مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Studying the association of LncRNA GAS6-ASI expression changes with EGER gene exon 21 mutations and its relationship with prognosis in Iranian colorectal cancer patients
Studying the association of LncRNA GAS6-ASI expression changes with EGER gene exon 21 mutations and its relationship with prognosis in Iranian colorectal cancer patients
elham siasi,1,*Nazanin Dabaghi,2Laiya Takbiri,3
1. Department of Genetic, Faculty of bioscience, Islamic Azad University, North Tehran Branch, Tehran, Iran 2. Department of Genetic, Faculty of bioscience, Islamic Azad University, North Tehran Branch, Tehran, Iran 3. Department of Cell biology, Faculty of bioscience, North Tehran Branch, Islamic Azad University, Tehran, Iran
Introduction: Colorectal cancer are a prevalent cancer type. Identifying biomarkers for prevention, diagnosis, and treatment is crucial. lncRNAs have emerged as potential biomarkers. This study aimed to compare the expression of lncRNA AS1-GAS6 and EGFR gene exon 21 mutations (G719A، L861Q and L858R) in both tumor and healthy tissues.
Methods: Fifty tumor and fifty healthy tissue samples were analyzed after confirmation by a specialist physician. RNA and DNA were extracted, and cDNA was synthesized. Real-time PCR was used to quantify AS1-GAS6 lncRNA expression and evaluated three mutations (G719A، L861Q and L858R) in exon 21 of the EGFR gene by PCR. The ABI system, Prism and SPSS software were employed for data analysis.
Results: In terms of gender distribution, the study included 26 males (52%) and 24 females (48%). The mean age was 60.80 ± 10.61 years, with an age range of 41 to 86 years. Gene expression analysis revealed a 2.08-fold increase in AS1-GAS6 lncRNA expression in tumor tissues compared to adjacent normal tissues. The analysis of EGFR gene exon 21 mutations showed a significant difference between the two groups regarding the presence of these mutations (p<0.05).
Conclusion: The results suggest that AS1-GAS6 lncRNA may serve as a novel non-coding RNA marker for Colorectal cancer diagnosis and prognosis. Additionally, the analysis of three main mutations in the EGFR gene exon 21 revealed that the L858R mutation could be a risk marker for Colorectal cancer patients.