مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Development of PEGylated alginate-Moringa-Tetracycline Composite Hydrogels: Physicochemical Characterization and In Vitro Assessment of Biocompatibility for Wound Healing Applications
Development of PEGylated alginate-Moringa-Tetracycline Composite Hydrogels: Physicochemical Characterization and In Vitro Assessment of Biocompatibility for Wound Healing Applications
Fatemeh Nikkerdar1,1,*Bahareh Jafari,2Saeedeh Samareh Moosavi,3Seyed Mohsen Saleh,4Saeedeh Askarian,5
1. Department of Biology, Kavian Institute of Higher Education 2. Department of Biology, Kavian Institute of Higher Education 3. Department of Biology, Kavian Institute of Higher Education 4. Department of Mathematics and Statistics, University of Neyshabur 5. Department of Medical Biotechnology, School of Medicine, Neyshabur University of Medical Sciences
Introduction: Hydrogels, as three-dimensional hydrophilic polymeric systems, offer distinct advantages over conventional wound dressings by maintaining a moist environment, minimizing tissue adherence, and enabling localized drug delivery. Chitosan, a naturally derived polycationic biopolymer, exhibits hemostatic, antimicrobial, and biodegradable properties favorable for tissue regeneration. Moringa oleifera extract has been recognized for its antioxidant and anti-inflammatory effects, while polyethylene glycol (PEG) enhances hydrogel flexibility and hydration. Additionally, tetracycline serves as a broad-spectrum antibiotic to control local infections.
Methods: Composite hydrogels were synthesized via chitosan and PEG, loaded with 10% w/w Moringa extract and 15% w/w tetracycline (relative to chitosan). Physicochemical assessments included swelling ratio, porosity, density, and degradation kinetics. Microstructural and chemical characterizations were performed via FE-SEM and FTIR analyses. L929 fibroblasts were used to assess cell viability (MTT assay) and wound closure capacity (scratch assay).
Results: FTIR spectra confirmed successful incorporation of Moringa and PEG via hydrogen bonding within the chitosan matrix. Moringa content was associated with decreased porosity and denser internal structures. All hydrogel variants maintained cell viability above 80%, indicating cytocompatibility. The formulation containing 10% Moringa exhibited optimal performance, with controlled degradation, moderate swelling behavior, and efficient antimicrobial delivery.
Conclusion: The PEGylated chitosan-Moringa-tetracycline hydrogel system demonstrates promising physicochemical and biological characteristics, supporting its potential use as a multifunctional wound dressing for enhanced tissue regeneration and infection control.