مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Design and Theoretical Formulation of a Latex Agglutination-Based Rapid Test Kit for C-Reactive Protein (CRP) Detection
Design and Theoretical Formulation of a Latex Agglutination-Based Rapid Test Kit for C-Reactive Protein (CRP) Detection
Milad Rahimi Ratki,1,*Zeynab Mohebbi,2
1. University of Science and Art, Yazd, Iran 2. University of Science and Art, Yazd, Iran
Introduction: C-reactive protein (CRP) is a widely used biomarker for inflammation and infection monitoring. The aim of this study was to design and theoretically formulate a latex agglutination-based rapid diagnostic kit for the semi-quantitative detection of CRP in human serum.
Methods: The kit components were conceptualized to include latex particles conjugated with monoclonal anti-CRP antibodies, a sample diluent, positive and negative controls, and a buffer solution.
Antibody conjugation was designed through covalent bonding to carboxylated latex beads to ensure antigen-antibody specificity and stability.
Results: Theoretical evaluations predict rapid agglutination within 2–5 minutes at room temperature, with visible results for CRP levels above 6 mg/L. Compared to existing methods such as ELISA and nephelometry, this kit offers a cost-effective, fast, and equipment-free alternative, making it particularly suitable for low-resource clinical settings.
Conclusion: The proposed kit is currently at the theoretical formulation stage and will be subjected to laboratory testing and validation in subsequent phases. If proven effective, it holds potential for commercialization as a knowledge-based product supporting point-of-care diagnostics.