مقالات پذیرفته شده در نهمین کنگره بین المللی زیست پزشکی
Identification of unique ZnT8 epitopes for antidiabetic vaccine design in three different servers
Identification of unique ZnT8 epitopes for antidiabetic vaccine design in three different servers
Masumeh Jalalvand,1Hamed Esmaeil Lashgarian,2Amirmasoud Jalalvand,3,*leila Abkhooie,4Maryam Zand,5Elahe yarahmadi,6
1. Assistant Professor, Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 2. Associate Professor, Department of Medical Genetics and Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 3. Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 4. Assistant Professor, Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 5. Department of medical biotechnology, Zanjan University of medical sciences, Zanjan, Iran. 6. Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Introduction: Introduction:
Type 1 diabetes is delineated by the obliteration of pancreatic β-cells, culminating in a complete deficiency of insulin that precipitates hyperglycemia. The pathogenesis of type 1 diabetes remains incompletely elucidated; however, it is widely acknowledged that the predominant form of the disorder is linked to autoimmune mechanisms (type 1A), and both genetic predispositions and environmental influences significantly contribute to the disease's onset (1, 2). ZNT8 is predominantly expressed within the pancreatic β-cells and has been recognized as a novel target autoantigen in individuals diagnosed with type 1 diabetes. Autoantibodies against Zinc transporter 8 (ZNT8) are identified in approximately 50-60% of patients experiencing acute-onset type 1 diabetes and in around 20% of those with a slow-onset form of the disease (3-5). Identifying the precise ZNT8 epitopes that are acknowledged by T lymphocytes in the configuration of epitope-MHC peptide complexes is essential for the advancement of tolerogenic vaccines designed to avert the autoimmune obliteration of pancreatic beta cells.
Methods: Methods:
The aim of this study is to identify the best epitopes for designing a vaccine capable of targeting ZNT8, an important autoantigen in type 1 diabetes. The amino acid sequence of ZNT8 was obtained from UniProt and used to determine MHC class II epitopes using the Immune Epitope Database (IEDB) server. In the epitope identification process, the common human alleles DRB1*04:01 and DRB1*03:01, each with a length of 15, were used.
Results: Result:
The best common antigen for ZNT8 was HKEVQANASVRAAFV, which was obtained from the IEDB server at http://tools.iedb.org/mhcii/ and the TEPITOOL server at http://tools.iedb.org/tepitool/ and syfpeithi at https://www.syfpeithi.de/bin/MHCServer.dll/EpitopePrediction.htm.
Conclusion: Conclusion:
A crucial first step in creating potent anti-diabetic vaccinations is identifying ZNT8 epitopes. By concentrating on these particular peptide sequences, we hope to avoid the development of type 1 diabetes by triggering a balanced immune response that encourages tolerance as opposed to autoimmunity. Additionally, because humoral autoimmunity is dynamic, ZNT8 in T1D will remain a study focus in order to create more precise diagnostic and treatment methods.