مقالات پذیرفته شده در پنجمین کنگره بین المللی زیست پزشکی
Evaluation of the efficiency of insulin-producing cells resulting from adipose tissue mesenchymal stem cell differentiation in glucose sensing and glucose stimulated insulin secretion
Evaluation of the efficiency of insulin-producing cells resulting from adipose tissue mesenchymal stem cell differentiation in glucose sensing and glucose stimulated insulin secretion
Dian Dayer,1,*Elham Ghalami negad,2 Seyed Javad Hosseini,3
1. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 2. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 3. Institute of Persian Gulf Research and Studies Center , Persian Gulf University (PGU), Bousher, I.R.IRAN
Introduction: Since insulin-producing cells resulting from stem cell differentiation cannot be as effective as pancreatic beta cells in regulating insulin-dependent glucose secretion, it seems necessary to identify the factors influencing this process. Therefore, this study was performed to investigate the changes in the expression of Glut2, ENO1, IGF-IR, Glucagon by insulin-producing cells resulting from the differentiation of adipose-derived mesenchymal stem cells.
Methods: Adipose-derived mesenchymal stem cells were differentiated into insulin-producing cells in a 14-day protocol using nicotinamide and ITS. The ability to produce insulin was confirmed by DTZ staining. Insulin and glucagon concentrations were determined by ELISA and the expression of Glut2, ENO1 and IGF-IR genes were measured by real time PCR.
Results: DTZ staining confirmed the presence of insulin secreting granules in insulin-producing cells. Differentiated cells secreted significantly more insulin and glucagon compared to undifferentiated cells. The differentiated cells showed significantly higher amounts of Glut2 and IGF-IR gene expressions compared with undifferentiated cells. While the expression of ENO1 was not significantly different between two groups.
Conclusion: The obtained insulin-producing cells exhibit the ability to naturally absorb glucose, perform glycolysis, and glucose-dependent insulin secretion in vitro.