مقالات پذیرفته شده در پنجمین کنگره بین المللی زیست پزشکی
Isolation and characterization of aquatic bacteria from the cold water of Mount Sabalan
Isolation and characterization of aquatic bacteria from the cold water of Mount Sabalan
tahereh ebrahimi,1vahideh tarhriz,2,*
1. Department of Nano-Biotechnology, Pasteur Institute of Iran, Tehran, Iran 2. Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
Introduction: Background: Cold environments are effectively inhabited by a large number of different psychrophilic bacterial species. The psychrophilic bacterial strains from these environments are a good supply for cold-active enzymes like proteases, amylases and lipases having industrial appliances especially in medical and pharmaceutical industries. Sabalan (Savalan) Lake is a stable crater lake locating at the summit of Sabalan. The biodiversity of microbial flora in this area needs to be explored to find its similarity with arctic regions biodiversity.
Objective: The psychrophilic bacterial population of Sabalan (Savalan) Crater Lake was identified. The current research is the first report of aquatic bacterial strains isolation and characterization from Sabalan Lake.
Methods: Water sample collections were cultured on four different media and colonies were isolated by the plating method. The phylogenetic features of isolates were examined and the phenotypic characteristics were investigated using specific culture methods.
Results: The results of morphological tests indicated that most isolates were Gram-negative and rod shape, which were able to grow between (-4)-(+37) ºC. The phyligenetic analysis revealed that the isolated strains belong to Pseudomonas, Yersinia, Kocuria, and Micrococcus genera and about 60% of the isolates belong to the various species of Pseudomonas as a dominant genus with abounded frequency
Conclusion: It can be concluded that the same cold environmental condition of these areas has the same microbial diversity with the same ancestors, while the geographical distances are so extensive.