مقالات پذیرفته شده در پنجمین کنگره بین المللی زیست پزشکی
The effect of diabetes disease on the expression of INSR & IRS-1 genes in infertile women
The effect of diabetes disease on the expression of INSR & IRS-1 genes in infertile women
Nader Namvarsigaroudi,1Zahra TahmasebiFard,2,*
1. Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran 2. Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Introduction: Introduction:
Infertility is a disease with complex disorders that occurs for a variety of reasons, some of which remain unknown. Infertility factors include a wide range of hormonal, immunological, physiological, weight, and physical activity factors. Genetic factors play a key role in reducing fertility. Balance of insulin hormone affects other important body hormones such as estrogen; progesterone and testosterone imbalances in these hormones have a wide range of side effects, including endometrial tissue and infertility.
Objective:
The purpose of this study was to investigate the expression changes of insulin receptor genes (INSR) and insulin receptor substrate (IRS-1) in the pathway of insulin signaling to endometrial tissue of infertile women compared between diabetic and Healthy women.
Methods: Materials and Methods:
In this study, thirty infertile women (4 diabetics, 26 healthy) who had been married for more than five years, an unknown cause of infertility, regular menstrual cycles, normal ovarian function, and absence of abnormalities in the uterus and fallopian tubes, or signs of endometriosis on ultra-sonographic or laparoscopic examinations were selected; in addition, the spouses of these people had normal volume and analysis of semen according to WHO criteria. Those who did not have this characteristic were excluded. Endometrial tissue of all women was prepared to evaluate the expression of INSR & IRS-1 genes using the Real-Time PCR method after RNA extraction.
Results: The results of this study showed that the parameters (Age & BMI) induced different changes in INSR and IRS-1 gene expression in both infertile subgroups and were statistically different. The Comparison of INSR & IRS-1 genes expression levels in endometrial tissue of healthy and diabetic individuals in each group showed a decreasing gene expression in the diabetic infertile woman and a significant difference between them. Healthy infertile persons showed 21.35 times more expression of the INSR gene than infertile persons with diabetes and 16.82 times more expression for the IRS-1 gene.
Conclusion: Diabetes affected the expression of genes and caused a reduction in the expression of both genes in persons with diabetes compared to healthy persons. His data can be one of the effective reasons for unspecified abortion. These genes are probability strong molecular markers for infertility.