Investigation of chloroquine effects on liver tissue in balb/c mice infected with plasmodium berghei

Taher Elmi,1,* Fateme hajialiani,2 Fateme tabatabaie,3 Mohamad reza asadi,4 Ali kalantari hesari,5 Fatemeh sadeghi,6

1. 1. Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2. 1. Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
3. 1. Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
4. 2. Ph.D in Histology, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organisati
5. 3. Ph.D in Histology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
6. 1. Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Abstract

Introduction

There are several families of drugs used to treat malaria. chloroquine (cq) which was first introduced in the 1940s and for many decades served as a cheap and reliable drug, is an anti-plasmodium drug that has many clinical uses in malaria treatment. chemical drugs that are prescribed to treat malaria disease currently have adverse effects such as diarrhea, vomiting and tissue damage, so it seems necessary to investigation of drug effects on vital tissues such as liver.

Methods

in this experimental study 40 mice were infected with plasmodium berghei and treated with chloroquine for four (4) consecutive days. toxicity testing was performed on liver mice. data were analyzed using spss software version 24 and analyzed statistically.

Results

The mean mice weight in groups treated with 50, 40, 20, 15 and 10 mg/kg of chloroquine was increased when compared with the positive control group and was decreased when compared with negative control group (uncontaminated mice treated with pbs), but it was not significant in any of the groups (p≥ 0/05). histopathological study of hepatic tissue showed no significant differences between negative control groups and experiment group in parameters such as apoptosis, cell necrosis, local inflammation and hyperemia. in the positive control group, the magnitude of the spleen was observed.

Conclusion

The results of this study showed that chloroquine does not have significant liver complications in the common therapeutic dose.

Keywords

Plasmodium berghei, chloroquine, tissue