Chemokines are related to sperm dfi in fallopian tube
Roudabeh Mohammadi,
1 Seyed omidreza mousavi,
2 Zahra zandieh,
3 Samaneh aghajanpour,
4 Marjan sabbaghian,
5 Reza aflatoonian,
6,*
1. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
2. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
3. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
4. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
5. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
6. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
Abstract
Introduction
Chemokines are one of the major compartments of immune system. it was showed that the chemokines have relevance in ovulation, sperm capacitation and fertilization. the human fallopian tube is an active assembly that undergoes the variation of chemokines. recently, the influence of dna fragmentation in sperm function has been interested for scientist. accordingly, in the current investigation the expression of different chemokines like il6, il8, il10 in human fallopian tube in presence of sperm with normal and abnormal dfi was evaluated.
Methods
Chemokine genes and proteins were evaluated in fallopian tube epithelial cells. sperm samples from 20 donors with normal and abnormal dfi were collected and co-incubated with fallopian tube epithelial cells. subsequently, the level of genes mrna expression was compared using rt-pcr in different groups. furthermore, the supernatant of different groups was applied for determining of the protein levels using elisa.
Results
Chemokine expressions, especially il6 and il8 in fallopian epithelial cells under the effect of normal sperm were higher than control group. also these genes significantly increased in abnormal sperm dfi. il10 was not expressed in control group. however, it is expressing in fallopian tubes at the presence of sperm (normal or abnormal dfi).
Conclusion
It seems that abnormal sperm dfi is established as a pathogen which leads the increasing of chemokines in fallopian tubes. thus, it can be hypothesized that interleukins are essential for sperm and fallopian tube interaction. this communication prepared safe environment for important events in fallopian tube like sperm preservation, fertilization and so on.
Keywords
Chemokine, sperm, dfi, fallopian tube
