Study of bcl-2 expression level in gastric adenocarcinoma cells which treated with rosa canina l extract
,1,* Dr.zahra deilami khiabani
,2 Dr.nader kazemi
1. Msc student ,Department of Genetic , Basic Science Faculty, Islamic Azad University, Zanjan Branch, Zanjan,Iran.
2. Assistant professor , Department of Genetic , Basic Science Faculty, Islamic Azad University, Zanjan Branch, Zanjan,Iran.
3. Assistant professor , Department of Microbiology , Basic Science Faculty, Islamic Azad University, Zanjan Branch, Zanjan,Iran.
Gastric cancer is recognized as the fourth most common cancer in the world, bcl-2 gene induces apoptotic pathway and is important gene in cancer studies. considering the least side effect of herbal extracts, in this study we have evaluated bcl-2 gene expression in ags cells treated with rosa canina l extract.
The ags cells were incubated 37°c containing 5% co2 with 85% humidity in dmem with 10% fbs. the cells were treated with concentrations of 800, 1200, 2000 μg /ml of rosa canina extract for 72 hours. extraction of rna, synthesis of cdna has been done using kit. the study of bcl-2 gene expression was performed by real time pcr and also gapdh gene was used as the internal control.
The results of real time pcr data show significant decrease in expression rate of bcl-2 gene in all concentrations of rosa canina l. in 72 hours treatment reduction of 1.48, 5.03 and 22.47 fold have seen in concentrations of 800, 1200, 2000 μg /ml of rosa canina l , respectively.
Bcl-2 may become therapeutic targets for cancer treatment and the herbal extract, rosa canina l, reduced the expression rate of bcl-2 significantly in dose dependent manner. this extract seems to have tremendous anticancer effects.
Bcl-2, rosa canina l, ags cells