Study of the production of paclitaxel and its antibacterial properties

Shamsozoha Abolmaali,1 Shamsozoha abolmaali,2,* Shakiba darvish alipour astaneh,3 Mehdi golestaninasab,4 Alireza asghari ,5

1. Semnan University
2. Semnan University, Semnan, Iran
3. Semnan University, Semnan, Iran
4. Semnan University, Semnan, Iran
5. Semnan University



Today's, secondary infections are problematic in cancer patients. therefore, evaluation of the anticancer drugs for their anti-bacterial features is reliable. paclitaxel is one of the most effective secondary metabolite against cancer which is extracted from yew trees in very trace amount. currently, researchers focused their attention on endophytes from yew as alternative resources for paclitaxel.


Previously, endophytic fungus from iranian yew were isolated in our laboratory and analyzed for taxol and or its derivatives production. trichoderma, ddfcc141, has been one of the candidates which further investigated for optimization of taxol compound production in mid medium. taxol extraction was analyzed by tlc and uv spectrophotometry in the companion with standard paclitaxel. in a time course test, the period of production was evaluated in 5, 10, 15, 20, 25 days. to confirm taxol (or its derivatives) production by ddfcc141, dbat gene was amplified by specific primer.


The expected 1700bp dna fragment was observed. according to standard curve of taxol, 605 ppm taxol was detected in mid and in 15 days of incubation. rf of standard taxol 0.91, baccatin 0.58 and purified taxol from ddfcc141 0.85 was obtained. the optimum time for extraction was within 15th days. the antibacterial evaluation showed a halo of about 20 mm against e.coli and proteus (sp).


More chemical and molecular investigations are needed to prove the potential of quercus endophytes for synthesizing taxan like compound.


Dbat gene, endophytic fungus, trichoderma, taxol, paclitaxel