Since,spherical particles(muraca ,2015) can caused intraction between immune system and epithelial cells and secretion cytokine and thus cause immune system maturity (ruiz*2016)
Methods
Standard strain was provided from takgenzist mrsagar, bloodagar culturs ,were used ,to grow the strain .
mrsbrothe was used for ineoculatiaon to grow to have culture en vrac. pcr(roche)was used 16srrna bifidobacter bifidum .
the concentration of pr was studied by nanodrap(thermos)
costant centerifugeues was used to extract extera cellular vesicle electerony microscoup was used to show ficicalc colifi cation and sds-page was used to detrmined chemical coalifications
Results
The band in 300pb shows that(figure1) this extense of bactry of this electerony microscop shows that the size of vesicaul are between 50-250nm(figure2) they are also esphericel sds-page result show that the size of vesicle are between 20-250 kdl(figyre3) that is in moreover,the prconcentration was 0.01mg/ml
figure2:electerony microscop figure1:band for pcr
figure3:band of sds-page
figure3:band of sds-page
Conclusion
Marco(2015) stated that the size of vesicle are between 50-250 that is the same as we achieved kim (2017)also stated that the size of vesicels in gram positive bacteria are between 20-250 that is in the harmony of our results.
acourding to the result to the study bifidobacter can be promising candidate in probiotic industry
