Immunotoxins are proteins that consist of an antibody fragment linked to a toxin, used as agents for targeted
therapy of cancers. although the most potent immunotoxins are made from bacterial and plant toxins, obstacles which
contribute to poor responses are immunogenicity in patients and rapid development of neutralizing antibodies. in the
present study we proposed a new therapeutic immunotoxin for targeted cancer therapy of ror1 expressing cancers:
an anti ror1 single chain fragment variable antibody (scfv)-endonuclease g (anti ror1 scfv-endog)
Methods
The three-dimensional structure of anti ror1 scfv-endog protein was modeled and structure validation tools were
employed to confirm the accuracy and reliability of the developed model. in addition, stability and integrity of the
model were assessed by molecular dynamic (md) simulation.
Results
All results suggested the protein model to
be acceptable and of good quality.
Conclusion
Anti-ror1 scfv-endog would be expected to bind to the ror1
extracellular domain by its scfv portion and selectively deliver non-immunogenic human endonuclease g enzyme as
an end-stage apoptosis molecule into ror1-expressing cancer cells and lead rapidly to apoptosis. we believe that anti
ror1 and other anti-tumor antigen scfv-endog forms may be helpful for cancer therapy.
Keywords
Cancer therapy- ror1- immunoconjugate- scfv- endog- apoptosis
