The frequency of e. coli isolates of urinary tract infections and their antibiotic resistance patterns in patients referred to imam khomeini hospital of shirvan during second semester of 2017

Moein Hamidi hesari ,1 Jafar hemmat,2,* Meead banitorfi,3

1. North khorasan university of medical sciences, Bojnurd,Iran
2. Biotechnology Department, Iranian Research Organization for Scientific and Technology(IROST),Tehran, Iran
3. Department of Microbiology, Sabzevar Islamic Azad University

Abstract

Introduction

Urinary tract infection (uti) is the most common extra-intestinal infection caused by enterobacteriacea bacteria. escherichia coli is the most common cause of urinary tract infection that causes 80 to 90 percent of urinary tract infections in young women. treatment of these infections has been difficult due to increasing antibiotic resistanses, therefore, selection of appropriate antibiotic has an effective role in controlling and treatment of the infection.

Methods

This cross-sectional study was performed on urine specimens in patients referred to imam khomeini hospital in shirvan during the second 6 months of 2017. strains were identified using biochemical tests including tsi, simmon citrate, sim, methyl red, etc., and differential culture media. antibiotic resistance pattern of the isolates was performed by kirby-bauer diffusion dispersion and on the muller hinton agar medium and according to the clsi guideline. the evaluated antibiotics were ampicillin, trimethoprim sulfamethoxazol,, cefazolin, cephalothin , gentamicin, norfloxacin and ofloxacin.

Results

out of 2395 studied urine samples, the culture of 112 (4.67%) samples were positive. a total of 72 urine samples were positive for escherichia coli (64.2%). out of the 7 antibiotics used, the most antibiotic resistance were related to ampicillin (72.5%), trimethoprim (57.7%), cefalotin (50%), cefazolin (48.3%), norfloxacin (31%), and the lowest resistance to gentamicin (11.6%).

Conclusion

: the study showed that escherichia coli is the most common cause of urinary tract infections and an ever-increasing increase in antibiotic resistance in e. coli bacteria. according to the study, gentamicin and ofloxacin are the best choices in treatment of urinary tract infections in this region of northeast of iran.

Keywords

Urinary tract infection, antibiotic resistance, escherichia coli