Gene expression comparison of myeloperoxidase in lung and liver tissues of septic rats treated with aspirin, celcoxib and indomethacin

Hamideh Salehiyan bahnamiri,1 Reza hajihosseini,2,* Saba miri,3 Azadeh rasooli,4

1. 1Department of Biotechnology, Faculty of Sciences, Payame Noor University, Tehran, Iran
2. 1Department of Biochemistry, Faculty of Sciences, Payame Noor University, Tehran, Iran 2
3. 3Department of Biotechnology, Faculty of Life Science, Alzahra University, Tehran, Iran
4. 1Department of Biochemistry, Faculty of Sciences, Payame Noor University, Tehran, Iran

Abstract

Introduction

Sepsis is a serious clinical condition that represents a patient’s response to a severe infection and has high mortality rate. for early detection of sepsis, biological marker such as myeloperoxidase(mpo)is usefull.non-steroidal anti-inflammatory drugs (nsaids), including aspirin,celcoxib and indomethacin have been frequently used for the clinical treatment of various inflammatory diseases.therefore, the effects of selected drugs on gene expression levels of mpo in lung and liver tissues were investigated.

Methods

Sepsis was induced in male wistar rats by using the cecal ligation and puncture (clp)¬. the animals were divided into6 groups. control¬: wild type, lap: with laparotomy surgery, clp,treatments: with clp and treated withnsaids (2 mg/kg b.w). after 48 hour, the rats were sacrificed,liver and lung tissueswere collected for gene expression comparisonof mpoby real-time pcr.

Results

In lap groupgene expression level of mpo increased compared to control group. in theclp group mpo gene expression increased more significantlycompared to other groups. when drugs was induced, the gene expression was dramatically decreased (p<0/05).aspirin in lung and celcoxib in liverhad milder effect in contrast with other drugs.it may due to asprin is a non-selective in¬hibitor of cyclooxygenase-1, celcoxib is a cyclooxygenase-2 specific inhibitor and indomethacin inhibits prostaglandin e2. as these genes are involved in inflammatory process, it may seem that they also affect on mpo gene expression.

Conclusion

Our study showed that the use of those drugs candecrease lung and liver damages via mpo inhibition.

Keywords

Sepsis, gene expression,myeloperoxidase,non-steroidal anti-inflammatory drugs