Preparation and characterization of superparamagnetic nanoparticles modified with pcl-peg copolymers containing cisplatin and its effectiveness evaluation on a549 lung cancer cell line

Sonia Fathi karkan,1,* Reza maleki baladi,2 Azizeh rahmani del bakhshayesh,3

1. Department of medical Nanotechnology, faculty of Advanced Medical Science, Tabriz Medical University, Tabriz, Iran
2. Young Researchers and Elite Club, Tabriz Branch, Islamic Azad University, Tabriz , Iran
3. Department of medical Nanotechnology, faculty of Advanced Medical Science, Tabriz Medical University, Tabriz, Iran



Magnetic iron oxide nanoparticles have become the main operational implements in biomedical and biological uses, and the mix of hyperthermia and controlled delivery of medicine is a favorable recent strength in cancer therapy. the purpose of the study was to examine whether cisplatin-encapsulated nano-particles enhanced the anti-carcinogenic impact of free cisplatin in lung cancer cells or not


Triblock copolymer pcl-peg-pcl was prepared using ring-opening polymerization of ɛ-caprolactone (cl) in the presence of poly (ethylene glycol). the bulk features of the copolymers were characterized by fourier transform infrared spectroscopy.cisplatin-loaded nanoparticles (nps) were prepared by double emulsion solvent evaporation technique and specified for drug entrapment efficiency(%), the content of medicine, size, and surface morphology. in vitro release outline of cisplatin-loaded np, formulations were specified. cytotoxic tests were evaluated in lung carcinoma (a549)-treated cells using mtt assay technique. furthermore, the particles were specified by scanning electron microscopy. fourier transform infrared spectroscopy and x-ray powder diffraction.


The anti proliferate effect of cisplatin performed much earlier when the medicine was encapsulated in magnetic nanoparticles compared to when it was free. cisplatin-encapsulated magnetic nano-particles improved the reduction in ic50 rate significantly. the in vitro cytotoxicity experiment revealed that fe3o4-pcl-peg magnetic nano-particles did not have cytotoxic effects and were biocompatible. the chemotherapeutic result of free cisplatin on cancerous lung cell is improved by its encapsulation in corrected magnetic nanoparticles.


This approach has the capability to defeat some main constraint of foreseeable chemotherapy and can be a desirable approach for future applications in the treatment of lung cancer.


Cisplatin- lung cancer- drug delivery