1. Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. 2. Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. 4. Zoonoses Research center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj,
back ground: mesenchymal stem cells (mscs) can be used to treat premature ovarian failure (pof).different methods have been used to detect mscs in tissues. the aim of this study was to investigate the quantitative distribution of cm-dii-labeled human umbilical cord vein mscs (hucv-mscs) in the ovarian tissues of the cyclophosphamide (ctx)-induced pof in the mice.
Methods: 40 adult female c57bl/6 mice were divided into four groups: 1- mice receiving pbs as control (ctrl) group. 2- mice receiving hucv-mscs intravenously as ctrl + hucv-mscs group. 3- mice receiving ctx intraperitoneally (ip) as ctx group. 4- mice receiving cm-dii-labeled hucv-mscs after ctx injection as ctx + hucv-mscs group. histological changes and cm-dii-labeled hucv-mscs distribution was analyzed in the ovarian tissues. also, quantitative real-time pcr (qpcr) was performed to detect human cytochrome b (cytb) dna in the ovarian tissues of the mice.
Results: the mean number of the fluorescent hucv-mscs was 20 ± 2.5 (57.1%) in the medulla, 11.3 ± 2.8 (32.2%) in the cortex and 5.5 ± 1 (15%) in the germinal epithelium of the ovarian tissue. moreover, human cytb dna was detected in the mice ovaries of the ctx + hucv-mscs group but was not detected in the other groups.
Conclusion: our findings suggest that the distribution of the transplanted hucv-mscs in different parts of the ovarian tissue was not equal and it was greater in the medulla than the cortex and germinal epithelium.