Evaluation of relationship between hypermethylation of dkk1 gene promoter with laryngeal squamous cell carcinoma

Arefe Mashhadi nezhad,1,*

1. Department of Genetics, Zanjan Branch, Islamic Azad University



Laryngeal squamous cell carcinoma is the second most common malignancy among head and neck cancers. several factors are involved in this cancer but epigenetic mechanisms are the most important factor in carcinogenesis. the most important epigenetic change known is methylation, which is divided into two types of hypermethylation and hypomethylation. the aim of this study was to investigate the status of methylation of promoter of dkk1 gene and its relationship with the prevalence and progression of laryngeal squamous cell carcinoma.


Dna was extracted from tissue samples of 29 patients. also, 30 healthy tissues were used as controls. extracted dna was treated by bisulphite and tested by methylation specific pcr. the results were analyzed by electrophoresis on agarose gel and spss software


Results:methylated, hem-methylated and non-methylated were found to be 13.79%, 75.86% and 10.34% respectively in cancerous tissue. methylated, hemi methylated and non-methylated were also found to be 0, 53.33% and 46.66% in normal tissue. also, there was a significant relationship between the status of methylation of patients and the degree of tumor differentiation (p = 0.04). overall, this study showed that methylation of promoter of dkk1 gene with laryngeal squamous cell carcinoma has a significant relation (p = 0.004)


The results suggest the role of the genetic factors in the incidence of laryngeal cancer and the methylation of the promoter of the dkk1 gene could be used as a biomarker in the prognosis and development of laryngeal squamous cell carcinoma.


Laryngeal squamous cell carcinoma. dkk1. hypermethylation