1. Azad University, Babol Branch, Department of Cellular and Molecular Biology, Iran 2. Azad University, Babol Branch, Department of Cellular and Molecular Biology, Iran
Bacterial urinary tract infections are found in all age groups associated with the development of strains resistant to antibiotic treatment. the aim of this study is to identify staphylococcus saprophyticus as a common cause of urinary tract infections through rrs gene by pcr and determining the antibiotic susceptibility of isolates of staphylococcus saprophyticus isolated from patients with urinary infections.
In this study, 51235 clinical samples were collected from patients with urinary tract infection from hospitals in mazandaran province for 8 months. identification of bacterial isolates were performed by biochemical tests and microbial susceptibility test to disk diffusion method according to the standard of clinical and laboratory standards institute (clsi). pcr test was used to determine the presence of rrs gene and confirmation of staphylococcus saprophyticus isolates identified by biochemical tests. 52 isolates (9.5%) of the 833 isolates of staphylococcus genus were identified as staphylococcus saprophyticus bacteria.
The results of the antibiogram test showed that the highest frequency of isolates resistance to antibiotics was related to erythromycin with 80% and the vancomycin, amycyline and cefotaxime antibiotics, respectively, with the frequency of %5/62, %5/5 and %5/16 were placed in the next ranks.
The present study shows that the bacterial resistance is a potential problem in iran and mazandaran province. therefore, monitoring of antimicrobial treatments and cheking the susceptibility tests in laboratory conditions can facilitate the control of the spread of drug-resistant microbes.