Swimming training by affecting the pancreatic level of sirt1 protein, improves metabolic conditions, and insulin sensitivity in type 2 diabetic male rats

Saber Ghaderpour,1 Rafighe ghiasi,2,*

1. 1 Department of Physiology, Tabriz University of Medical Sciences, Tabriz- Iran 2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz-Iran
2. 1 Department of Physiology, Tabriz University of Medical Sciences, Tabriz- Iran 2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz-Iran

Abstract

Introduction

Type 2 diabetes is a metabolic disorder, which characterized by insulin resistance and a disturbance in the metabolism of lipids, carbohydrates, and hyperglycemia. recent studies have been recommended that defect and reduction of sirt1 (a nad-dependent class iii histone deacetylase) activity may be related with metabolic diseases such as atherosclerosis and t2dm. this study aimed to evaluate the effect of swimming training on the pancreatic level of sirt1 protein, metabolic conditions, and insulin sensitivity in type 2 diabetic male rats.

Methods

28 male rats were allocated into four groups: control (con), exercise (exe), diabetes (dia) and diabetic exercise (dia- exe) (n=7). diabetes induced by a high-fat diet (hfd) for 4 weeks and a low dose of stz(35 mg/kg, ip). 7days after confirming of diabetes, the rats swam in the exercise groups for 12 weeks. at the end of the intervention and after the sedation with ketamine and xylazine, the blood samples and pancreatic tissue was prepared for biochemical measurements and pancreatic levels of sirt1 protein.

Results

This study indicated a significant decrease (p<0.01, p<0.05) in the pancreatic level of sirt1 protein and hdl, quiki (quantitative insulin sensitivity check index) and a significant disturbance (p<0.05) in the metabolic parameters (lipid profile and fbs). swimming training significantly (p<0.01, p<0.05) increased the pancreatic level of sirt1 protein and improved the metabolic parameters and insulin sensitivity.

Conclusion

These results suggested that swimming training by increasing the pancreatic level of sirt1 protein improved insulin sensitivity and metabolic disorder. these study findings suggested that the content of the expression of sirt1 in pancreatic tissue may be a promising novel therapeutic target for diabetic complications.

Keywords

Diabetes; swimming training; sirt1; insulin sensitivity