Strong impact of helicobacter pylori oipa gene on the development of peptic ulcerations (pus) in iran

Omolbanin Feili,1,* Saber zahri,2 Saeid latifi-navid,3 Seyedeh zahra bakhti,4

1. Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, 56199-11367, Iran
2. Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, 56199-11367, Iran
3. Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, 56199-11367, Iran
4. Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, 56199-11367, Iran

Abstract

Introduction

Helicobacter pylori (h. pylori) is recognized as a causative agent of peptic ulcerations, gastric adenocarcinoma, and low-grade mucosa lymphoid tissue (malt) lymphoma. the blood group antigen-binding adhesion (baba) and outer inflammatory protein (oipa) involved in h. pylori adherence to gastric epithelial cells have been suggested to have a role in the pathogenesis of gastrointestinal disease. we here aimed to clarify the roles of the h. pylori baba2 and oipa genotypes in development of severe gastrointestinal disease.

Methods

Genomic dna was extracted from h. pylori -positive cultures gastric biopsy specimens and genotyped by pcr. a simple logistic regression was used to analyze association between variables. p<0.05 was considered statistically significant.

Results

Frequency of the baba2+, baba2-, oipa+, and oipa - genotypes in patients with pus was 25.0%, 75.0%, 97.7%, and 2.3%, respectively, and in non-atrophic gastritis group (nag) was 34.7%, 65.3%, 63.9%, and 36.1%, respectively. there was no significant relationship between presence of baba2 with pus and nag. results of the logistic regression analysis showed that the oipa+ genotype was strongly correlated with the risk of pus, the or was 24.304 [95% confidence interval (ci), 3.251-181.677, p = 0.002].

Conclusion

The oipa+ genotype of h. pylori could be a strong biomarker for risk prediction of pus in iran.

Keywords

Helicobacter pylori, peptic ulcerations, baba2, oipa, iran.