Zno nanoparticles effect on viability and chromosomal stability of spermatogonial stem cells

Maryam Farzaneh,1 Azam javadi,2 Saadat mokhtar,3 Seyede faezeh moraveji,4 hamid gourabi,5,* Fereshteh esfandiari,6

1. 2. Department of Developmental Biology, University of Science and Culture, Tehran, Ira
2. 2. Department of Developmental Biology, University of Science and Culture, Tehran, Ira
3. 3. Department of Physics, Shahid Beheshti University, Tehran, Iran
4. 1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
5. 4. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
6. 1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

Abstract

Introduction

Zinc oxide (zno) nanoparticles (np) are being widely used in various industries, such as in cosmetics, food additives and biomedical applications. therefore, it serves as a part of our daily life. however, there is a serious lack of information about potentioal toxicity of zno nps on livingorganisms. recent studies have shown that administration of nps can easily pass through the blood – testis barriers and affect the male reproductive system; in this system spermatogonial stem cells (sscs) are critical for the process of spermatogenesis and disruptions in sscs function lead to infertility. therefore, the aim of this study to investigate the potential toxicity of zno nps on the sscs.

Methods

In this regard, we synthesized zno np and characterized them by xrd and sem. sscs have been isolated from nmri mice testes and were characterized by immunofleurescence (if) analysis. then, sscs treated with different concentrations of zno nps (10, 20, 30, 40 and 50 μg/ml).the viability of the cells was assessed by pi staining. then, on days 1 and 7 after treatment, the chromosomal aberrations and aneuploidy analysis was assessed by giemsa and g banding method respectively.

Results

Xrd and sem analysis confirmed successful synthesis of zno nps. the results of this study showed that the survival of treated cells with zno np decreased significantly compared to the control group at the concentration of 20 µg/ml and other higher concentrations. also, the entry of nps into the cell was confirmed using an electron microscope (tem). moreover,, we observedsignificant increase in chromosomal aberrations seven days after treatment with zno nps (p <0.05). there was no significant difference aneuploidy following the treatment.

Conclusion

Taken together our data demonstrate that zno nps have genotoxicity efects in sscs by inducing chromosomal aberrations. this study indicated that cytotoxicity of zno nps is dose and time dependent.

Keywords

Spermatogonial stem cells, zinc oxide nanoparticles, chromosomal aberrations, aneuploidy.