2. department of biology,Islamic Azad university of Roudehen branch, Roudehen, Iran. 3. Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Abstract
Introduction
Breast cancer is the most common cancer in the world and is the second leading cause of death in women. among the predisposing factors of cancer, genetics is one of the major factors affecting other factors, such as the environment and nutrition. tumor-sensitive tumors in breast cancer, including brca1, make women more susceptible to breast cancer. in this study, the relationship between rs1799950 polymorphisms of brca1 gene and breast cancer in iranian women was investigated.
Methods
100 patients with breast cancer and 100 normal samples were selected. all of the specimens were captured and their dna was extracted by salting out. after confirmation of purity of dna, the sequence was amplified by pcr technique and cut off with specific enzyme. then the genotypes of the results were determined by the number of cut bands. the results were determined using spss software and x², logistic regression, and so on
Results
The gg mutant genotype was not significantly associated with the two groups (p value: 0.082, or: 1.746, ci95%: 0.930-3.281). this genotypic mutant has a significant relationship with body mass index (p-value: 0.004) and histopathologic changes (p-value: 0.0001). however, with age (p-value: 0.331) and grade of cancer (p-value0.147) the relation of the term does not indicate meaning.
Conclusion
Genetic change from a to g in position 356 of brca1 gene (rs1799950) is not related to the risk of breast cancer in iranian patients.
