Evaluation of four phenotypic methods for the detection of carbapenemase producing pseudomonas aeruginosa and their comparison with the polymerase chain reaction
Increase resistance to carbapenems is a worldwide clinical concern.early and correct identification of carbapenemase-producing isolates for management of antimicrobial therapy is very important.we compared the performance of the modified hodge test (mht),imp/edta , carba np test (cnpt) and carbapenem inactivation method (cim) for quick and precise carbapenemase enzymes diagnosis.
The methods were appraised by using 97 pseudomonas aeroginosaisolates, the collection contained 48 non-carbapenemase, 11 klebsiella pneumoniae carbapenemases (kpc) producers, 19 verona integron-encoded metallo-β-lactamase (vim) producers, 20 imipenemases (imp) producers, 35 oxacillinases (oxa48) producers, and 25 strains harboring ampc carbapenemase genes.
During this study, 47 carbapenem-resistant pseudomonas aeroginosa isolates were subjected to carba np ,mcim,mht,imp/edta tests, and tested by pcr for blakpc, blavim, blaoxa48, blaspm, blasim, blagim, blaampc, blaimp genes .48/49 (97/95%) isolates were positive for carbapenemase production by carba np,46/49 (93/87%) by mcim , 27/49 (57/44%) by using ddst and 25/49 (%) by mht .
Our results showed that carbanp test, carbapenem inactivation method (cim) test are very are highly sensitive and proprietary, brummagem, prompt methods for the discovery of carbapenemase producer isolates. and helpful instrumentation which can be accomplished in the routine laboratory method for diagnosis of carbapenemase producing in p. aeruginosa isolates.
Evaluation, phenotypic methods, carbapenemase, p. aeruginosa