1. MSc of Biotechnology at Shahid Bahonar University of Kerman, Iran. 2. Associate Professor of Biotechnology, Shahid Bahonar University of Kerman, Iran.
Abstract
Introduction
Liposome is one of the nanocarriers that allows the loading of lipophilic and hydrophilic drugs. the cumin(cuminum cyminum l.) has an anti-cancer effect, an increase in the metabolism of respiratory diseases. herbal essences have limited application due to instability and low solubility. the goal of this study was to develop nano-liposomes containing essential oil of cumin as one of the nanocomposites of the drug and to evaluate their anticancer effect on the cell line of mcf7 for use in modern drug delivery systems.
Methods
The cumin(cuminum cyminum l.) essential oil was first extracted and then gas chromatography and mass spectrometry were used to identify the extracted compounds. thin-film hydration method was then used to create small liposomes loaded with the extract. the lipid phase was prepared with l-alpha phosphatidylethanolamine dioleoyl, cholesteryl hemisuccinate and the essential oil. finally, the cell toxicity of liposomal and free essential oil was compared on mcf7 cell line.
Results
The employed methodology produced nanoliposomes measuring 243.6 nm in size with 100% frequency and polydisparity index of 0.33. cell toxicity assay showed 13% increase in cancer cell death with liposomal essential oil.
Conclusion
In this study, nanoliposomes containing cumin(cuminum cyminum l.) essential oil were produced. cell toxicity assay showed increased cancer cell death of liposomal essential oil compared to the free essential oil.
Keywords
Nanoliposomes; drug delivery; essential oil; cumin(cuminum cyminum l.) and mcf7.
