Effect of hyaluronic acid produced with recombinant streptococcus zooepidemicus created by wild type on osteoarthritis

Alireza Zakeri,1,*

1. Department of Biology Science, , Shahid Rajaee Teacher Training University, Tehran, Iran



Hyaluronic acid (ha) is responsible for the viscoelastic properties of synovial fluid which inhibits the loss of proteoglycans from the matrix of joint cartilage. it is reported that the intra-articular injections of ha can compensate for osteoarthritis (oa) inflammation induced loss of lubrication in joints. this would relieve oa associated symptoms by alleviating pain and irritation. herein, we aim to evaluate the oa treating properties of a novel ha source.


We have a native wild strains of streptococcus zooepdemicus and by genetic manipulation have created a recombinant strain. genetic manipulation wild strain of bacteria causing the production of hyaluronic acid with other properties and clear that the rheological properties, the molecular weight of ha and its concentration are factors which could determine the intra- articular viability of the moleculer. a novel source of ha was produced by recombinant bacterial strain , injected into the joints of inducted oa model rats. then, developing a novel scoring scheme which the percentage based cartilage degeneration score (cds) reports are replaced by an actual micrometer based scaling concept, the extent of the treatment success was assessed.


It is indicated that the novel ha introduced in this study is of oa treatment potential equal to a commercial counterpart


Since the bacterial strain of the employed ha is engineered for high concentration ha fermentation, this ha could be contemplated as a novel cost effective oa treatment source. moreover, the devised quantitative method of assessing the extent of oa cartilage damage could be employed in similar studies.


Osteoarthritis, hyaluronic acid, cartilage degeneration, recombinant streptococcus zooepidemicus