Hybrid gelatin/poly (glycerol sebacate)(pgs) electrospun membrane as a potential wound dressing
,1,* Anooshe zargar kharazi
,2 Jaleh varshosaz
1. Department of Advanced Medical Technology, Biomaterials Group, Isfahan University of Medical Sciences
2. Department of Advanced Medical Technology, Biomaterials Group, Isfahan University of Medical Sciences
3. Department of pharmaceutics, school of pharmacy and Isfahan pharmaceutical science research center, Isfahan University of Medical Sciences
When the skin as a physical barrier is damaged, pathogens have a direct route to infiltrate the body, possibly resulting in infection. therefore our task is primarily treatment the wounds to prevent the infections and stimulate the skin to repair is in second grade. nowadays biodegradable materials are gaining extensive attention in the field of soft tissue engineering
gelatin is a biodegradable and non-antigenic polymer, which provide hemostasis and facilitates cell adhesion and proliferation during healing process.
poly(glycerol sebacate)(pgs) is a synthesis polyester which is biocompatible, biodegradable, inexpensive and generally has soft and flexible mechanical properties.
in this study we fabricated the gelatin / pgs blend scaffold with ciprofloxacin as an antibiotics drug by electrospinning method for preventions the infections and skin tissue engineering
Sebasic acid and glycerol combined in 1:1 ratio at 120°c under nitrogen gas and high vaccum for 24 hours to prepare the pgs synthesis polymer. after synthesis process gelatin added to pgs in 3:1 ratio and %25 (w/v) of polymers solved in %80 (v/v) acetic acid in 37~ 40°c stirring for 3 hours. at the end 0.025 gr of ciprofloxacin added to the solution.
for the electrospinning process, a 5 ml syringe was used to inject the polymer solution by flow rate of 0.5 ml/h and 12cm distance between the needle and collector of device, while the voltage was kept at 18 kv.
finally scaffolds crosslinked by n,n-(3-dimethylaminopropyl)-n0-ethyl-carbodiimide hydrochloride (edc) and n-hydroxysuccinimide (nhs) in 2.5:1 ratio in %90 ethanol which is less cytotoxic compared to glutaraldehyde.
the surface morphologies, fiber diameter, prosity and inter connectivity of the electrospun scaffold, before and after crosslinking process, were characterized using scanning electron microscopy (sem) and fourier-transform infrared spectroscopy (ftir) being used to verify the chemical composition of the scaffold and study the specific interactions between gelatin and pgs. for antiobiotic test we done the disk diffusion method and for cell viability of the scaffold we handle colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-di¬phenyltetrazolium bromide (mtt) assay.
all the resulte about the rate of drug releasing obtaned by uv-visible spectrophotometry.
The fiber size after crosslinking increase from 178±60 to 872±90 nm which might be due to swelling of the fibers during the crosslinking process. prosity of crosslinked membrane increased from 80.49±0.32 to 87.92±0.05 and three prosity layer of scaffold showed the interconnectivity before and after crosslinking process which determined by matlab software.
ftir analysis showed the characteristic peaks of gelatin, pgs and ciprofloxacin without any additional peaks of infections before and after crosslinking process. the antimicrobials test demonstrated different degrees of antimicrobial activity against both negative and positive bacterial species evaluated and cell viability assay in 24-well culture plate showed the reduction of mtt. the spectrophotometry results showed the %50 percent of drug releasing in first 24 h after soaking in pbs(buffer phosphate salin) and %70 in first 72 h.
Our results showed that crosslinked gelatin/pgs membrane with controlled release of ciprofloxacin, could be a promising biodegradable membrane for wound dressing and regenerative medicine by prevention of wound infections in first stage.
gelatin, poly (glycerol sebacate), ciprofloxacin, skin tissue engineering, drug delivery