1. Pasteur institute of Iran 2. Pasteur institute of Iran 3. Pasteur institute of Iran 4. Pasteur institute of Iran
Abstract
Introduction
Sand fly saliva contains proteins with modulating host immune system and plays an important role in both blood feeding and outcome of leishmania infection. the profile of salivary proteins were examined and analyzed from different endemic foci of p. papatasi by focusing on endemic antigens for vaccine production.
Methods
Sand fly specimens were caught from endemic area of zoonotic cutaneous leishmaniasis in northern khorasan province, iran by sticky papers and cdc light traps. different methods of protein extraction were employed and a new technique was developed because of lack of enough number of specimens. proteins were extracted from salivary gland tissue with a lysis buffer. purification was performed using reverse phase hplc with a linear gradient protocol from 40 to 60% of acetonitrile.
Results
Sds-page revealed 15 separated bands ranging 11-275 kda. the protein of ppsp15 was isolated with 15kda weight in 60% acetonitrile. among sp15 like proteins, ppsp15 is the first report of a very high hydrophobic protein of salivary glands from iranian p.papatasi.
Conclusion
For vaccine production against leishmaniasis, local salivary gland proteins and efficient extraction method should be intended. depending on locations, choosing different and suitable proteins should be considered.
